Recommended Texts:
- Fundamentals of Biochemistry, by Donald Voet, Judith G. Voet; Charlotte W. Pratt; 2016.
- Principles of Instrumental Analysis (7th Edition), by Douglas A. Skoog, F. James Holler, Stanley R. Crouch, Brooks Cole; 2017.
- Introduction to Proteomics: Tools for the New Biology, by Daniel C. Liebler; 2001.
- Introduction to Proteomics: Principles and Applications, by Nawin C. Mishra; 2010.
Description: The total number of human protein products is close to one million. Numerous studies have investigated the functions and regulations of these proteins. Most of these studies fall within the domain of proteomics, which is the large-scale study of the structure and function of proteins. Proteomics has become a critical component in identification of disease-specific biomarkers used in diagnostic and prognostic tests. Subsequent analytical separations of proteins and peptides are performed by chromatographic techniques, such as Liquid Chromatography (LC) or Capillary Electrophoresis (CE) or Mass Spectrometric (MS). The Course focuses on learning the structure of proteins and peptides, using analytical techniques to separate and identify them, and using protein data bases to analyse the obtained data.
Proposed Lecture:
(Note: topics covered may be modified as necessary)
- What is proteomic?
- Amino acids and proteins
- Protein digestion
- Medical proteomics
- Clinical proteomics
- Application of proteomics
- Extracting proteins from biological samples
- Methodology of separation and identification of proteins
- Proteomics, protein modification
- Proteomics, future development